The role of hormone treatment on complete Regeneration of Jashir (Prangos ferulacea Lindl) in vitro

Prangos ferulacea (L.) Lindl (Apiaceae) is a permanent and aromatic plant grows wild in some regions of Iran. There are not any reports about plant regeneration of P. ferulacea in vitro. In the present study, the effect of various concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D) with kinetin and naphthalene acetic acid (NAA) with Benzyl amino purine (BAP) on shoot and plant regeneration of P. ferulacea radicle, hypocotyl, crown, and cotyledon explants were investigated. The study was designed as a completely randomized design with 20 treatments and 3 replications. The best results of shoot regeneration was recorded case of crown explants in MS medium comprising 0.5 mg/L 2,4-D with 1mg/L Kin and 0.5 mg/L 2,4-D with 1.5 mg/L Kin treatments (67%, in each cases). The regenerated shoots were rooted on basal MS medium without any hormonal treatment.