Study of Sheep PROP1 Gene Polymorphism Using PCR-RFLP

PROP1 (Prophet of POU1F1) is specifically expressed in the pituitary gland. The PROP1 gene organizes three exons encoding for the 226 amino acids in humans and domestic animals; this gene is localized on chromosome 1 and regulates growth hormone، prolactin، and thyroid. The aim of this research was to study the polymorphisms of the PROP1 gene in Zel، Lori-Bakhtiari and Zel-Atabay crossbred sheep breeds، using PCR–RFLP. Blood samples from 90 Lori-Bakhtiari (research station)، 60 Lori-Bakhtiari (slaughterhouse)، 90 Zel (research station) and 40 Zel-Atabay crossbred (slaughterhouse) sheep were collected. DNA was extracted using the salting out method and the desired fragment was amplified using PCR. The genotypic frequency of GG and GA in Lori-Bakhtiari (research station)، Lori-Bakhtiari (slaughterhouse)، Zel (research station) and Zel-Atabay crossbred sheep were 0. 98%، 0. 02%، 0. 86%، 0. 14%، 0. 96%، 0. 04%، 0. 89%، and 0. 11%، respectively. The genotypic frequency of AA and AB in Lori-Bakhtiari (research station)، Lori-Bakhtiari (slaughterhouse)، Zel (research station) and Zel-Atabay crossbred sheep were 0. 70%، 0. 30%، 0. 78%، 0. 22%، 0. 68%، 0. 32%، 0. 84%، and 0. 16%، respectively. It can be concluded that polymorphism of this gene can be used as a candidate gene for some biometric traits.