The study of electrophoresis pattern, ribonuclease enzymes activity and investigation of S-RNase gene in self- and cross-pollinated styles of cultivars Petunia hybrida Bravo cool water mix and P. hybrida Bravo purple star

In Petunia hybrida، Self-incompatibility is controlled by stylar glycoproteins. These glycoproteins could be able to recognize and reject self-pollen. These proteins have ribonuclease activity. In this study، the activity and isoenzyme pattern of S-RNases in self and cross-pollinated styles of Bravo purple star (self-incompatible) and Bravo cool water mix (self-compatible) were investigated (spectrophotometically and PAGE electrophoresis، respectively). The expression intensity of S-RNase was also studied in self- and cross-pollinated styles. Because of the importance of S allele in self-incompatibility interactions، the S-allele was identified in both cultivars. Self-pollinated styles of Bravo purple star showed the highest activity of RNases. Different isoenzyme pattern of S-RNases was found in the styles of two cultivars. The results showed that S-RNase is expressed in both self- and cross-pollinated styles of both cultivars. But، the highest expression was observed in self-pollinated styles of Bravo purple star (self-incompatible). By the comparison of DNA sequencing of S-RNase in Bravo purple star to other identified S-RNases، it was found 100% identity of this cultivar S allele to S3-RNase in Petunia hybrida sub species inflate،. There was 100% identity between nucleotide sequence of S-RNase in Bravo cool water mix and allele S1-RNase in Petunia hybrida.