Association of DD Genotype of Insertion/Deletion Polymorphism of Angiotensin-Converting Enzyme Gene with Systemic Lupus Erythematosus and Lupus Nephropathy

Systemic lupus erythematosus (SLE) is a multisystem disease with unknown etiology. We hypothesized that insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) gene may influence the development and/or progression of SLE and lupus nephritis. In a crass sectional case-control study, genomic DNA from 106 SLE patients and 103 healthy controls matched for sex, age, and ethnicity, were genotyped for the (I/D) polymorphism of ACE gene by polymerase chain reaction (PCR). Comparison of quantitative variants between two groups was assessed by student t-test and association between qualitative variables was analyzed by the chi-square or Fisher exact tests. The frequency of DD genotype in SLE patients was significantly higher than control group (25.5 % vs. 14 %), and the risk of SLE was 2.2 times greater in subjects with DD genotype than the individual by DI and II genotypes (OR, 2.2 [95% CI, 1.1 to 4.4]; p=0.023). The distribution of D allele in SLE patients was significantly higher (p=0.021) compare to controls (47 and 36.4, respectively). The Risk of nephropathy in SLE patients with DD genotype was three times more than other genotypes (OR), 3 [95% CI, 1.1 to 8]; p=0.027]. This study demonstrated that ACE DD genotype acts as a risk factor on SLE and Lupus nephropathy in an Iranian population.