The Considering of Histological Changes of Liver in Cholestatic Rats in Different Time Periods

In cholestasis changing bile acids and salts and opioids is associated with trauma، fibrosis and necrosis liver tissue. For examination of liver truma advance، this study shows time effects of closing bile duct on rat liver histological changes.

Cholestasis was induced in male Wistar rats by bilateral ligation of bile duct and cutting. Animals were divided to four groups (Sham، 7، 13 and 21 days after cholestasis). Rats were killed under anesthesia and immediately their livers were dissected. The specimens were processed routinely and were sectioned into 5 microns thickness slices. The sections were stained by Hematoxiline-Eosin (H&E) method and then studied using optical microscope.

Seven days after cholestasis، hepatocyte necrosis and cell disarrangements were observed in liver sections. Thirteen days after cholestasis، necrotic cells were increased and wrinkle chromatic nuclei were seen. Twenty-one days after cholestasis widespread necrosis was seen. Nuclei condensation and bill duct reproduction were observed. Also cell wall disappeared and tissue was out of order.

Data showed that after disease progression and accumulation of bill salts and acids، expansion of necrosis and fibrosis were rapid، and creation of cirrhosis is a good reason for rapid recognition and treatment of the cholestasis.