Effect of heat stress on in vitro culture of ram Sertoli cell

The present study was conducted to evaluate some oxidative stress in ram Sertoli cells under heat stress condition. The testis of 10 ram lambs was gathered from abattoir (during 5 times) and transferred to laboratory for isolation and culture of Sertoli cells. Isolated cells from lamb testes were divided into 3 plates and maintained in temperatures 32 ºC (control), 39 ºC (mild stress) and 42 ºC (severe stress) for 6 hours. The percentage of cell viability, the amount of lipid peroxidation (TBARS), the antioxidant capacity (FRAP) and superoxide dismutase activity were measured in all groups after 6 hours. The percentage of alive cells were reduced in group 42 ºC (78 ± 6.8) as compared to control (95 ± 9.3; P<0.05). Lopud neroxidation was higher in sever stress (0.6 ± 0.06) than control (0.46 ± 0.05; P<0.05). In conclusion, heat stress could be a detrimental factor for viability of Sertoli cells via increasing of lipid peroxidation.