Trichoderma reesei endoglucanases increase by butanol concentration method

Cellulase products usage in industry is in demand considerably. This enzyme is used in paper, paint, oil and textile industries. Cellulase can convert cellulose to glucose and then glucose can be converted to ethanol which this process can be replaced with fossil fuels. Cellulose is a polysaccharide that can be renewable and may be in future providing fuel for transportation. Cellulase converts cellulose to soluble sugars. One of the most extensively studied organisms is the soft rot fungus Trichoderma reesei. This fungus produces a complete set of cellulolytic enzymes that are able to cleave β- 1, 4- glycosidic bonds present in cellulose or cellulose derivatives. One of the key enzymes in the multi- enzyme complexes is endoglucanase which is purified from different method. By using the butanol to concentrate this enzyme, we obtained remarkable active enzyme. The amount of cellobiohydrolases are much more than the concentration of endoglucanases (mentioned by several articles) in Trichoderma reesei. In this experiment, we could obtain the concentration of endoglucanases 20% more than cellobiohydrolases.