The Effect of Β-casein Nanoparticles on Bioavailability and Cellular Uptake of Platinum Complex as an Anticancer Drug

Due to the low solubility and high toxicity of drugs، treatment of cancers is problematic; therefore، the encapsulation and targeted delivery of therapeutic effect is required. The aim of this study was to investigate the effect of nanoparticles on cellular uptake and bioavailability of beta-casein on platinum complexes as cancer drugs. In the present experimental study، the physicochemical properties of nanoparticles as drug carriers of beta-casein devices using dynamic light scattering (DLS) and scanning electron microscopy (SEM) were investigated. In order to evaluate the toxicity effects of platinum complexes، the colon cancer cells in the absence or presence of free platinum complex concentration and nanoparticle loaded with platinum complexes were incubated for 24 and 48 hours. LD50 Values (concentration of compound causing 50% mortality in the cells) was determined using the MTT assay. Data were analyzed by ANOVA and post-hoc test. At a concentration of 1 mg ml، beta-casein nanoparticle drug carriers were synthesized in the range of 100 to 300 µM. In addition، the mortality rate in cancer cells by the release of platinum complexes (without and with the capsule)، were 70 and 26 in 24 hours، and 60 µM and 21 µM in 48 hours respectively، The study showed that the bioavailability of the encapsulated platinum complexes increases and new drug delivery system may be a good candidate for the treatment of cancer.