Comparative evaluation of cytotoxicity of four endodontic sealers using human gingival fibroblasts

Biocompatibility is one of the most important properties of endodontic sealers. The aim of the current study was to investigate the cytotoxicity of four endodontic sealers (AH26، MTA Fillapex، tgsealer and ADSEAL) on human gingival fibroblasts in vitro. In this in vitro experimental study، four endodontic sealers were evaluated in fresh and set states. The fresh and set specimens were separately extracted in RPMI-1640 (Sigma-Aldrich، USA). The extracts were placed for 1، 3 and 7 days in close contact with C165 human gingival fibroblast in cell culture. Then the samples cytotoxicity was assessed by MTT assay (3-(4،5-dimethylthiazol-2-Yl) -2،5-diphenyltetrazolium bromide) and spectrophotometric analysis. Data were analyzed with multivariate analysis of variance using SPSS 11. 5 (α=0. 05). The results showed that all the sealers were cytotoxic. According to statistical analysis a significant difference was seen between fresh and set specimens in AH26 (p value = 0. 028) but there were no significant differences in ADSEAL (p value = 0. 910)، MTA Fillapex (p value = 0. 952) and tgsealer (p value = 0. 566) between the two sealer states. In addition، based on means of all the data of each sealer MTA Fillapex (0. 78) had the least cytotoxicity and ADSEAL (0. 60) had the highest cytotoxicity. It can be concluded under the limitations of the present study that among the sealers evaluated in the present study MTA Fillapex and ADSEAL had the highest and lowest cytotoxicity، respectively.