Cloning, expression and immunological activity analysis of a ScFv from mouse monoclonal antibody against human plasminogen capable of enhancing fibrinolysis

Nowadays، monoclonal antibodies are the most applicable biological drugs. To that end، the construction of ScFv is the first step، having the advantage of small size، rapid clearance from blood، and better penetration to the tissues. A monoclonal antibody (A1D12) that enhances the activation of plasminogen 3-50-fold in the presence of plasminogen activators is a good candidate as a drug for coronary disorders. The main goal of this study is to construct the single chain fragment of variable (ScFv) for humanization. In this investigation ScFv of A1D12 has constructed and expressed in E. coli، which is capable of recognizing the corresponding antigen according to the results of SDS-PAGE and competitive ELISA respectively.