Effect of 50Hz Electromagnetic Fields on P448 and P450 Cytochromes and Gonadal Steroid Hormones in Male Mice

Due to the increasing and widespread usage of the variety of electric and magnetic devices and possible effect of electromagnetic fields caused by them on human health many researchers have focused to investigate the harmful effects of these fields. This study aimed to investigate the effects of 50Hz uniform electromagnetic fields on P450 and P448 cytochromes and gonadal steroid hormones in male NMRI mice. The study was done on 12 adult male NMRI mice. The mice were randomly divided into two control and experimental groups including 6 animals. To produce the uniform electromagnetic field solenoid cramps systems were used. The experimental group was exposed to 50Hz magnetic field with an intensity of 0.06mT for 28 continuous days and consecutive 4hour for each day and the results were compared with control group that was not exposed to the field. After blood sampling, LH, FSH and testosterone levels were measured by Gamma counter then liver was removed to study the P448 and P450 cytochromes. LH level increased in experimental group than control group and testosterone level decreased that these changes were significant statistically (p<0.05). FSH level had not significant change in the experimental group (p>0.05). Also mutagenic effects of electromagnetic fields were led to P448 and P450 cytochromes change. The 50Hz electromagnetic fields can affect gonadal steroid hormones and P448 and P450 cytochromes in male NMRI mice.