Effect of heparin on sperm fertility, oocyte,s fertilization and tow cell embryos in NMRI mouse in vitro

Heparin is used by researchers to enhance sperm fertility and embryo development. In vitro fertilization in mouse is recognized as a standard test for assisted reproduction studies. In order to improve this test, in the present study the effect of M16 and T6 medium with heparin (10ug/ml) and without heparin were investigated on fertility parameters of sperm and oocyte including, Sperm number, motility and survivability rate, oocytes fertilization rate and embryo development to tow cell stage in NMRI mouse. Results for effect of heparin in two medium treated and control groups show fertilization rate inT6 media (p<0.001) and zygotes development rate to two cell embryos in M16 media (p<0.001) were significantly different. Comparing also effect of these two medium for motility and survivability of sperm, M16 media (p<0.05) and for oocytes fertilization rate and two cell embryo production, T6 (p<0.001) was better media. However, M16 especially with heparin is better than T6 media (with and without heparin) for increment of sperm motility and survivability, and T6 with heparin is more suitable than T6 without heparin and M16 (with and without heparin) for obtaining a better in vitro fertilization rate in NMRI mouse.