Consideration and Selection of Suitable Hormonal Composition for in vitro Shoot Regeneration and Propagation of Ocimum basilicum L.

Ocimum basilicum L. (sweet basil), from Lamiaceae family, is rich in aromatic essential oils and valuable in industry for its pharmaceutical, aromatic properties. The present study has focused on tissue culture of O. basilicum using leaf explants from in vitro germinated plants. The current study has been fulfilled based on completely randomized design with 5 replications using MS (Murashige and Skoog) medium with different concentrations of BAP (0, 1 mgl-1) and 2,4-D (0,0.5,1mgl-1). After callus formation the calluses were transferred on new MS media with (1, 3 & 5 mgl-1) BAP in combination with 0.2 mgl-1 NAA, for regeneration. The highest average number of shoots occurred in the medium containing 1 mgl-1 BAP and 0.2 mg-1 NAA. The presence of NAA inhibited root formation, when combined with different concentrations of BAP.