The effects of henna leaf alcoholic extract on angiogenesis of chorioallantoic membrane and some embryonic morphological abnormalities of chick embryos

Angiogenesis, formation of new vessels from the primary vessels is involved in physiological condition such as reproduction, wound healing and pathological like diabetes and tumor. Tumor progression associates with persistent unregulated angiogenesis. This study aimed to investigate the effect of alcoholic extract in henna (Lawsonia inermis) leaf on the angiogenesis of chorioallantoic membrane and morphological abnormalities of chick embryos. In this experimental study, 40 fertilized eggs of Hy-line race were randomly divided into 4 groups: control group, sham-exposed group and two empirical groups (n=10). In the second day of incubation in sterile condition, a window was opened on eggs. On 8th day, chorioallantoic membrane (CAM) was treated at sham-exposed group with normal saline and at empirical groups with alcoholic extract in henna (Lawsonia inermis) leafs (50, 200 mg/kg). On 12th day CAMs, the length and weight of vessels were examined and morphological disorders were studied. Data were calculated and analyzed statistically using t-test and ANOVA. Weight and length CR of chick embryo at the control group with the sham-exposed group showed no significant difference in the mean comparison of number and length of vessels. The mean of number and length in the second empirical group and in the first empirical group at the comparison of vessel's length and the CR length than the control group showed significant decrease (P<0/05). Variables mean in two empirical groups showed no significant difference. Morphological abnormalities were not observed in fetuses. According to the results of this research, alcoholic extract of henna leaf inhibits angiogenesis on chorioallantoic membrane, but it had no significant effect on chick embryo's morphological characteristics.