Evaluation of Expression of Pluripotency Markers in ES-like Cells derive from Spermatogonia Stem Cells and Embryonic Stem Cells

Recently، attention has been diverted to the pluripotency characteristic of Spermatogonia Stem Cells (SSCs) in in-vitro culture. The aim of this study was to evaluate the morphological and molecular genetics changes of differentiated SSCs to Embryonic-like Stem cell (ESC). The SSCs were collected from neonatal mouse testis via a two-step mechanical and enzymatic digestion and cultured in DMED containing 15% FBS. ES-like cells colonies was appeared in the fifth passage. The expression of pluripotency and spermatogonia specific genes; Nanog، SOX2، Klf4، Stra8، DAZL، Plzf as well as the expression of puripotency markers Oct4 and Alkaline Phosphatase Activity (ALP) of ESC respectively was investigated using RT-PCR and immunocytochemical techniques and compared with ESCs. The pluripotent characteristic of ES-like cells derived from SSCs was confirmed based on morphological investigation and high expression of ALP as well as pluripotency marker Oct4. The expression of specific spermatogonia genes like Stra8، DAZL، Plzf was decreased and the expression of pluripotency genes such as Nanog، SOX2، Klf4 was increased during the differentiation of SSCs to ES-like cells. In vitro culture of SSCs was conformed to induced ES-like cells. This process was accompanied by wide alteration in molecular profile expression of specific spermatogonia and pluripotency genes. In over all، SSCs can be considered as an opening window to generate pluripotent stem cell.