Discrimination and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer SFF-SFR

Message:
Abstract:
Self-incompatibility in almond and Prunus species is an important genetic trait that prevents self-fertilization. Self-incompatibility in almond is controlled gametophytically by the multiallelic S-locus. This study was done in order to identification and preliminary selection of self-compatible progenies resulted from controlled crosses in almond using specific primer SFF-SFR.Some important morphological traits of parental crosses were evaluated using almond descriptor. Also, offspring’s (F1) of five crosses including; A (Tuono × 101 Genotype), B (Supernova × 101 Genotype), C(Genco × Shahrood 21), D (Tuono × Shahrood 12) and E (Tuono × shahrood 17) were tested using PCR in order to DNA amplification and self-compatibility evaluation. Results of PCR method found that self-compatible progenies showed 449 bp band, while this band not observed in self-incompatible progenies. In addition, all self-incompatible progenies appeared no S1 allele at any condition.
Keywords:

almond , self , incompatibility , Sf , allele , S1 , allele , SFF , SFR primer , PCR

Language:
Persian
Published:
Journal of Applied Crop Breeding, Volume:1 Issue: 2, 2013
Pages:
111 to 122
https://magiran.com/p1318753  
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