Impact of the Anthropogenisation on the Metal Bioaccumulation and Distribution in the Spiny-Cheek Crayfish (Orconectes limosus Raf.) from Lake Gop3o, Poland

This study was undertaken to determine the concentrations of metals in the muscle and exoskeleton of the crayfish Orconectes limosus in order to compare them with other species of crayfish and crustaceans. In addition, we analyzed the correlation between the concentrations of the same element in the exoskeleton and the meat and different elements accumulated in the same tissue. Crayfish individuals were acquired in spring (May 2012) from Lake Gop³o. Individuals with damaged claws were not analyzed. Due to the relatively low amounts of meat that was obtained from the abdomen of individual crayfish, material from individuals with a similar body length was combined; thus, there were ten samples of meat and the exoskeleton obtained on the whole. The muscle samples (about 12 g) were freeze dried in a Finn-Aqua Lyovac GT2 freeze dryer (Finland). Three replicates of each freeze-dried sample were acid digested with 1 ml of concentrated HNO3 and 1 cm3 H2O2. Metal analyses were performed using an inductively coupled plasma mass spectrometry (ICP-MS) with a Perkin-Elmer Optima 8300 spectrometer. The analyses revealed that the mean metals concentrations (mg·kg-1) in the meat of the crayfish and in the exoskeleton were in the following order (Zn (115.571) > Mn (18.825) > Cu (17.226) > Ni (15.472) > Pb (3.535) > Cr (0.769) > Co (0.551) > Cd 0.315) > Hg (0.138)) and (Mn (111.640) > Zn (11.355) > Ni (8.165) > Pb (6.695) > Co (0.595) > Cu (0.575) > Cd (0.379) > Cr (0.195) > Hg (0.0168)), respectively.