Rapamycin Enhances HBV Production by Inducing Cellular Autophagy
Author(s):
Wenjuan Huang , Fengrong Zhao , Ying Huang , Xia Li , Sufei Zhu , Qin Hu , Weixian Chen *
Abstract:
Background
Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained..Objectives
In this report, we studied the mechanism by which rapamycin enhances HBV replication and expression by inducing cellular autophagy..Materials And Methods
HepG2.2.15 cells were treated with rapamycin to induce autophagy. Autophagosomes were observed by fluorescence microscopy and transmission electron microscopy. Autophagy marker protein LC3-Ⅱ/LC3-Ⅰwas detected by Western blotting. HBV DNA and mRNA were determined by real time PCR and Southern blotting. HBsAg was evaluated by ELISA..Results
In HepG2.2.15 cells, HBV DNA and HBsAg increased when host cells were treated with rapamycin and the effect was reversed by autophagy inhibitor, 3-methyladenine (3-MA)..Conclusions
These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin..Language:
English
Published:
Hepatitis Monthly, Volume:14 Issue: 10, Oct 2014
Page:
6
https://magiran.com/p1328524
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