Investigation of Aspects of In Vitro Regeneration In African Violet (Saintpaulia ionantha Wendl,) and Its In Vivo Rooting

In this study، the microproliferation of leaf explants in African violet was evaluated as several experiments، independently. Different stages of experiments was included study of the best way to disinfect of surface، establishment medium of explants، determination of the most suitable concentration of hormone for shoot regeneration and proliferation. also induction of rooting in vivo was done using different media. The results showed that the best treatment for explants disinfection were 70 % ethanol for 30 seconds with 1 % sodium hypochlorite for 10 minutes. The highest percentage of shoot induction and proliferation was obtained on MS medium supplemented with 2 mgL-1BA. In rooting stage، in vitro rooting was removed، and regenerated plantlets were successfully rooted in vivo conditions. The best medium for rooting was Peat- Perlite powder with the longest root and the most survival. after establishment of the plantlets، they transferred to soil in natural conditions and were kept in the greenhouse.