Invitro survay on effect of root Methanol extract of two floral plants of Azarbaijan province, Iran (Ferula szowitziana and Ferula rigidula) on promasigote of Leishmania infantum

Leishmania infantum is responsible for visceral leishmaniasis regarded as one of the health problems of over than 88 countries. Drugs used to treat leishmaniasis have undesired side effects or they are not effective at all. Considering the prevalence of leishmaniasis disease، it is necessary to produce an effective، safe and inexpensive drug. Different species of ferula have antibacterial، antifungal and anti-Leishmania features. However، no study has been conducted on the effects of extracts of plants including Ferula rigidula and Ferula szowitsiana on promastigote of Leishmania infantum parasite. Two species of Azerbaijan province indigenous ferula were collected and their chloromethane and methanol extracts were prepared. Promastigotes of Leishmania infantum were cultivated at RPMI cultivation in vitro environment and the effects of the mentioned extracts on growth and survival of promastigotes of Leishmania infantum were evaluated using homocytometer slide under a microscope. Methanol extract of both ferula species with different concentrations were successful in controlling promastigote dose-dependent growth of Leishmania infantum. All outcomes resulted from verifying the extracts using homo cytometer slide. According to the results of the study، Ferula rigidula and Ferula szowitziana indigenous species of Iran demonstrated their appropriateness to study leishmaniacidal effects in vitro. Therefore، it is required to further study and identify the effective compounds found in methanol extract of the studied species.