Genetic and phylogenetic analysis of Mitochondrial DNA control region of Khorasan native chicken

Study on native chickens could be considered as a way to maintain population genetic variation, breeding programs development and helps to obtain information on their genetic structure. Mitochondria genome Sequencing is one of the most practical methods in order to determine phylogenetic relationships among close populations and species. The aim of this study was to investigate the nucleotide sequence of the mitochondrial DNA control region of Khorasan native chickens, its genetic and phylogenetic analysis. Blood samples were supplied from 5 Khorasan native chickens. Then 845 bp fragments of extracted DNA were amplified by using specific primers. Sequencing of amplified fragments was carried out with Sanger method. Using of similar sequence of mitochondria DNA from other chicken breeds available in the NCBI database, phylogenetic tree was drawn and matrix of genetic distances was formed between Khorasan native chickens and other breeds for mitochondrial DNA control region. According to the results no variation was observed among the nucleotide sequences. Phylogenetic test results showed the least genetic distance was recognized between Khorasan native chicken with Marandi chicken of Iran, White Leghorn, Buff Cochin and satsuma dori of Japan, Lv erwu of China, Denizli and Gerze of Turkey, Dandarawi of Egypt and India. Thereby can conclude that Khorasan native chicken of Iran has a close relationship with Asian chickens.