Hematological and serum biochemical profile of rainbow trout (Oncorhynchus mykiss) fed immunogen

Current use of prebiotic as indigestible feed additive improves health statues of host، instead of drug and chemical material in aquaculture. The aim of this experiment was to investigate the effect of 2g. kg-1 prebiotic immunogene on hematological، biochemical and some serum enzymes parameter of rainbow trout after 7 weeks feeding. Hundred and twenty rainbow trout (81. 65 ± 1. 49 g) After checking the health status and acclimatized to laboratory condition for 10 days، they were equally stocked in six fiberglass tanks (1000 L) assigned to two levels (0 and 2 g kg-1) of Immunogen (commercial prebiotic، ICC Co; USA) with three replicates. During the experimental trial (7 weeks) fish were hand-fed (2% of BW) twice daily (09:00 and 17:00). At the end of trial the blood was collected using a hypodermic syringe from the caudal blood vessels and was used to evaluate the hematological and biochemical parameter. The results of this experiment indicated that some hematological parameter such as RBC، Hb، MCH، MCV and MCHC were not affected by immunogen (p>0. 05)، but the Hct and WBC were increased in rainbow trout immunogen (P 0. 05). Considering serum biochemical parameter it was observed that in the group fed with prebiotic immunogen increased the level of glucose (P<0. 05)، Although the level of triglyceride and serum total protein were not affected، but the level of serum cholesterol was increased in group fed with prebiotic immunogen (P>0. 05). The results indicated that administration of immunogen no effect on ALT، AST، LDH and ALP levels. These results indicate that fish blood parameters and serum biochemical profiles could be affected by immunogen، which should be taken into account in future studies.