Effect of Salicylic Acid and Salt Stress on Seed Germination and Functional Antioxidant in Broccoli Sprout

Sulforaphane is a strong anticarsenogen compound which is produced in broccoli. It seems salt stress improves sulforaphane production via systemic acquired resistance. To obtain suitable salt concentration and for having more content of sulforaphane with controlling germination percentage، NaCl in 5 levels 0، 50، 100، 150، 200 mM (0، 4. 53، 9. 125، 13. 68، 18. 25 dS/m) and salicylic acid in 3 levels)0،100، 200 µM(were examined. A logistic regression model was used to assess the effects of treatments on the maximum cumulative germination percentages، rate of increase، and germination lag times. Sulphoraphan and vitamin C were determind by HPLC method. Combination between salicylic acid and chloride sodium improved sulforaphane concentration. The 100 µM salicylic acid with 100 mM chloride sodium was the best treatment as the highest concentration of sulforaphane and vitamin C with perfect growth factors achieved in the treatment.