Improved isolation purple mebrane contain bacteriorhodopsin from Halobacterium salinarum (Article Uncorrected Proof)

Message:
Abstract:
Nowadays، biological substances have allocated many applications to themselves in distinct industries. In this field، biological molecules with various potentials have been identified، from which we can refer to bacteriorhodopsin (BR). Bacteriorhodopsin is found in purple membrane of halobacterium salinarum. Due to its stability and various characteristics like possession of properties of a proton pump، bacteriorhodopsin has many applications in different industries. One of the most important industrial and semi-industrial production processes for bacteriorhodopsin is the isolation and purification of the purple membrane. In this investigation، after halobacterium salinarum culture، the purification was done according to the Yucel method. So as to produce bacteriorhodopsin in semi-industrial scale، a modified method was developed by substitution of mechanical approach with enzymatically method to destruction of DNA and uses of osmotic shock instead of dialyze. This method led to decrease of time and isolation cost in comparison to Yucel method. The contamination percentage of the PM was estimated below 5% for both methods. The purification percentages were 67±1% and 68±4% for the modified method and Yucel method، respectively، which is indicating of the equal purification percentage for both methods. Bacteriorhodopsin amount was 8. 2±0. 4، 8. 1±0. 6 mg per liter for the improved method and Yucel method، respectively. The enzyme activity assay by Kuyama method indicated that the pH variation was 1 unit with the same BR amount for both methods. Hence، the modified method introduced in this investigation could reduce time and costs of the purification by maintaining the BR characteristics.
Language:
Persian
Published:
Modares Journal of Biotechnology, Volume:4 Issue: 2, 2013
Pages:
39 to 48
https://magiran.com/p1370536  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!