Isolation of a fragment of a new MYB transcription factor gene in mulberry(Article Uncorrected Proof)

One of the largest gene families in plants is MYB which contain a DNA-binding domain regulating gene transcription. These transcription factors (TF) have various functions in a wide range of plants. For example، biosynthesis pathway of anthocyanin as secondary metabolites and responsible for coloration in different plant tissues is regulated by the above-mentioned TFs. In the current study، Using homologous genes in the species close to mulberry (Morus Alba)، a fragment of a new MYB transcription factor belonging to MYB gene family was isolated from this plant. First protein sequence of 14 different genes regulating anthocyanin from the species close to mulberry family (moraceae) were selected and then aligned، subsequently the region with the highest similarity was nominated. Following this، the cDNA sequence of these genes was aligned and a location which had a high rate of similarity was considered. With regard to large polymorphism in this sequence، degererate primers were designed and used to isolate the target gene.