Examining Antithrombotic Property of Extracting Glycosaminoglycan Compositions from Carcharhinus dussumieri Whitecheek Shark's Cartilage(Article Uncorrected Proof)

Sharks are relatively large sea creatures by an extensive cartilaginous skeleton. The shark cartilage is a rich source of bioactive molecules including collagen protein and glycosaminoglycan. In the present study، Cetyl Piridinium Chloride cationic salt was used for extracting of glycosaminoglycans from dryed cartilage of Carcharhinus dussumieri and their anticoagulant properties were examined. FTIR spectrum was also used to identifing and structurally compare with heparin. The total amount of the extracted glycosaminoglycan was 42. 8 mg/g of the dry cartilage. Also، FTIR spectrum results confirmed the presence of heparin- like compounds in the extract. Finally، the anticoagulant properties of extracted glycosaminoglycans was examined by the Activated Partial Thromboplastin Time anticoagulant test (APTT) method in 410، 763، and 1250 concentrations، and Prothrombin Time (PT) method in the 1250 concentration on the human plasma. The anticoagulant time was 43، 50، and 85s in 410، 763، and 1250 concentrations of extracts، respectively، which extended the coagulation time 1. 3، 1. 5، and 2. 5 folds.