Optimization of direct and callus mediated regeneration of Jatropha curcus through in vitro organogenesis

The Jatropha curcas L.، a plant belongs to Euphorbiaceae family، which has great economic and medicinally value. There is high amount of oil in seeds of this plant. Because of low germination ability of seed، propagation of Jatropha curcas via seed is not usual. The purpose of this work is optimizing of Jatropha curcas tissue culture for optimization of callus formation and propagation through micropropagation. The whole experiments were performed in complete Split- Factorial block design with nine replicates. In the current work the effect of different concentrations of 6-benzyladenine (BA) and indol-3-butyric acid (IBA) hormones (0، 0. 25، 0. 5، 1، 1. 5، 2، 3 mg/lit) in MS medium were investigated on callus and shoot formation on leaf، petiole stem and node explants. The results showed that the combination of BA and IBA is more effective for callus formation. The highest percentage of callus formation (90. 63%) was observed at 2 mg/lit BA and 1 mg/lit IBA in stem explants. The results showed that the highest percentage of shoot induction was observed at 0. 5 mg/lit BA and 0. 25 mg/lit IBA in node explants. Meanwhile our observations indicated that the root regeneration was happened equally at different concentrations، but the highest regenerated root number were observed after 0. 5 and 1mg/lit of IBA treatment. Finally، the obtained plants were transferred into soil medium.