Regeneration of White top (Cardaria draba L.) using Tissue Culture

Tissue culture is a useful and applicable method in micropropagation and plants mass production in a short period of time irrespective of growing and planting season. Meanwhile it is a new and effective method to breed plants through gene transformation. Despite these advantages, a useful tissue culture system for effective regeneration is required for many plants such as White top (Cardaria draba). White top contains a lot of sulforaphan for which it is considered a medicine plant which in turn highly requires a suitable protocol for its effective regeneration. In this study, the samples gathered in Kerman Township were used and the ability of the plant for callus induction and shoot regeneration was studied in vitro in two separate factorial experiments in randomized complete block design, BAP: in 7 levels and NAA in 4 levels. The first experiments were carried on cotyledon explants and the second one on hypocotyl explants with 3 replications in the years 2010 and 2011. Considering the statistical analysis (p≤0.05), 3 mg/L BAP plus 0.5 mg/L NAA treatment and 3 mg/L BAP plus 1 mg/L NAA hormone treatment had the callus induction and the most suitable number of shoots for cotyledon and hypocotyl explants, respectively. The best root generation was for cotyledon and hypocotyl explants using only 1 mg/L NAA treatment and 0.5 mg/L BAP plus 1 mg/L NAA treatment, respectively. All the samples were placed on MS liquid containing 2 mg/L IBA and then they were elongated in the full strength medium without growth regulators for 2 weeks. After that, they were kept in the pots containing sterilized peat moss under greenhouse conditions for 4 weeks which resulted in 90% success in the plants regeneration.