Cloning and Phylogenetic Relationships Analysis of an Oxidoreductase Gene in Product Pathway of Ethylene Hormone

1-aminocyclopropane-1-carboxylic acid oxidase (ACO) enzyme is a member of the Fe (II-dependent family of oxidases/ oxygenases) and for activity requires Fe2+ as cofactors and ascorbate as a co-substrate. This enzyme is the only oxidoreductase in the ethylene biosynthesis pathway and catalysis the biosynthesis terminal step. In the present study, total RNA was extracted from tomato fruit (cv, Memory I), and then, coding cDNA of the LeACO1 gene was synthesized by reverse transcription polymerase chain reaction (RT-PCR). The PCR product was cloned into a pUC19 plasmid, and then, their biochemical, structural and phylogenetic characteristics were analyzed. LeACO1 amino acid sequence studies in tomato and other plant of such family were indicated that His177, His234 and Asp179 amino acids are formed a triangle catalytically site to play a role in binding Fe2+ cofactor. Phylogenetic and multiple alignment analysis were illustrated a high degree of identity to those of the ACO1 gene from other plants.