No association between recurrent implantation failure (RIF) following in vitro Fertilization (IVF) with gene Polymorphism (P53 Arg72Pro)

Message:
Abstract:
Introduction

recurrent implantation failure (RIF) is the most common cause of pregnancy failure after in vitro fertilization (IVF). Among the different causes of RIF, the role of maternal genetics factors is as one of most important factors. P53 codon 72 polymorphism is one of the notable polymorphisms in this field. According to evidences about the association between P53 polymorphism and increased risk of RIF in different population, if this relationship be justified in Iranian population, this polymorphism can be used to predict IVF success rate. This study was performed with aim to evaluate the association between this polymorphism and incidence of RIF.

Methods

This case-control study was performed on 80 infertile couples in Mashhad Montaserieh Infertility Treatment Center in 2010. The infertile couples were divided into three groups of control, RIF-1, and RIF-2. The control group included 40 women who were successfully pregnant after IVF. The patient group 1 were 20 women who no type of pregnancy was observed after two IVF, and patient group 2 were 20 women who failed to be pregnant after at least three IVF. P53 gene polymorphism genotype (Arg72pro allele) was determined using PCR- ARMS technique. Statistical analysis was performed by SPSS software (version 16) and Pearson Chi-Square test. P<0.05 was considered significant.

Results

The frequency of Arg/Pro genotype in control group, patient group 1, patient group 2 and total patients was 92.5%, 75%, 80% and 77.5%. The frequency of Arg/Arg genotype in these groups was 7.5%, 25%, 20% and 22.5%, respectively. Pro/Pro genotype was observed in none of the groups. According to Pearson Chi-square test results, distribution of genotypes was not significantly different between patient group 1 and patient group 2 and also total patients in comparison with control group.

Conclusion

According to the results of present study in the studied population, there is no association between P53 codon 72 polymorphism and occurrence of RIF. So this polymorphism cannot be used to predict the risk of RIF.

Language:
Persian
Published:
Iranina Journal of Obstetrics Gynecology and Infertility, Volume:18 Issue: 172, 2015
Pages:
18 to 25
https://magiran.com/p1492710  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!