The effect of glucose and glutamine supplementation on serum HSP72 in nonathlete men during four weeks exhausting endurance-intermittent training

The aim of the present study was to investigate the effect of glucose and glutamine supplementation on serum HSP72, in non-athlete men during four weeks exhaustive endurance - intermittent training that causes glycogen depletion. For this purpose 20 non-athlete healthy men were selected and randomly divided into four groups including: glucose supplementation with glycogen depletion training group (n = 5, supplement 1), glutamine supplementation with glycogen depletion training group (n = 5, supplement 2), glycogen depletion training group (n =, placebo) and a group without any treatment (n = 5, control). The blood samples were collected at the onset of training protocol period and 48 hours after final training session from anticubital venous. Serum HSP72 concentration were determined by ELISA technique. The main and interaction effects of variables were determined using TWO WAY ANOVA compeleted with Tukey post-hoc test. The significant level was chosen as α = 0.05.The results showed the significant effect of training variable for serum HSP72 concentration, [F(1,17)= 6.362 P<0.05]. The effect of supplementation variable was significant for serum HSP72 concentration, [F(1,17)= 15.362 P<0.01]. Post-hoc test showed the significant difference between placebo with supplement 1 group (p<0.05) and placebo with supplement 2 group (p<0.01). The interaction effect between two variables was not significant. In summery, the results suggested that in glycogen depletion training condition, glucose or glutamine supplementation have significant effect on serum HSP72 concentration.