Optimization of phenazine 1- carboxylic acid extraction by Pseudomonas Aeruginosa MUT.3

Replacing toxic antifouling paints with coatings that have no effect on the marine environment is an important issue. Phenazine 1-carboxylic acid (PCA) is one of the effective antibiotics, which can be used as antifouling agent in marine coatings to prevent attachment of marine species on ship hulls. In this study, Phenazine 1-carboxylic acid (PCA) as an antibacterial agent which produced by Pseudomonas Aeruginosa MUT.3 was investigated. PCA extraction process was optimized using Response Surface Methodology (RSM). Results showed that at 30 min mixing time, 150% of solvent and using ethyl acetate as solvent are considered the optimum levels and factors in phenazine 1-carboxylic acid production which produced by P. aeruginosa MUT.3. In the present paper, the optimization of extraction process suggested as an effective way to increase the production in biological processes in which the extraction of PCA increased by 34% compared to previous reports.