Effects of Different Mediums, Sucrose and NAA to Rescue of Embryos Produced by the Cross Between Two Lily Species in Ovary slice culture Method

In interspecific hybridization of lilies¡ resulted embryos are aborted because of the incompatibility. Therefore applying the embryo rescue methods are very essential. The main purpose of this research is introducing a suitable medium to rescue embryos produced by the cross between two lily species¡ (L.cavales) and (L.Casablanca). In the first test¡ during 10¡ 15 and 20 days after pollination (DAP)¡ ovary slices were cultured on OSC medium contained NAA (1 and 0.1 mg/l) and sucrose ( 6 and 9 percent)¡ that 10 DAP¡ 6% sucrose and 1 mg/l NAA were significant in increasing the growth percentage. In the second test¡ the ovary slices ovules were separated based on the culture times and then transferred on different mediums¡ each one contained two levels of sucrose ( 3 and 6 percent ) that 6% sucrose were significant in increasing the germination. Obtained Data from tow expriment was set up as a factorial based on randomize complete design.