Effects of aqueous extract of Holothuria arenicola and low frequency electromagnetic field on serum insulin, glucose and beta-amyloid (Aβ1-42) in diabetic rats

Beta-amyloid is increased in the brain of diabetic patients. Considering different effects of electromagnetic field on biological processes, this study was performed to investigate the concomitant effects of aqueous extract of Holothuria arenicola and low frequency electromagnetic field (EMF) on the serum insulin, glucose and amyloid beta level in diabetic rats.
In this experimental study, 42 male rats were divided into 7 equal groups: control and diabetic control (distilled water); diabetic 1(100 mg/kg of extract); diabetic 2 (100 mg/kg of extract EMF 10 gauss); diabetic 3 (100 mg/kg of extract EMF400); diabetic 4 (EMF10); diabetic 5 (EMF400). Diabetes was induced by intra-peritoneal injection of Alloxan. Diabetic groups 2, 3, 4 and 5 had daily exposure to the EMF for 60 minutes. The serum levels of insulin, blood glucose and beta-amyloid protein were measured at first, fifteenth and thirtieth days.
The serum glucose, insulin and beta-amyloid level for diabetic samples 1 and 2 at first, fifteenth and thirtieth days were significant compared to the diabetic control. At fifteenth and thirtieth days, glucose and insulin level for diabetic 3 and 4 samples were significant compared to the diabetic control (P Injecting Holothuria arenicola extract to the diabetic rats results in a decrease and increase in glucose, insulin and beta-amyloid, respectively. These effects were augmented with the simultaneous use of 10 gauss electromagnetic field.