Protein pattern of Yersinia ruckeri isolates from farmed rainbow trout (Onchorhyncus mykiss)

Yersiniosis is one of the most important fish bacterial diseases cause by of Yersinia ruckeri. Isolation, characterization and of protein profile of Yersinia ruckeri were studied in some affected rainbow trout farms in Iran. The kidney and spleen samples from 16 fish farms in Charmahal-va-Baakhteyiari, Mazandaran and Qazvin provinces were cultured on blood agar at 220C for 48 hours and the grown bacteria were characterized by phonotypical and molecular studies. Biochemical and molecular works resulted in identification of six isolates of Yersinia ruckeri. The whole proteins of the six recovered isolates of Yersinia ruckeri subjected to sodium dodecyl sulphat - poly acrylamide gel electrophoresis (SDS-PAGE) showed that the protein profiles of 5 isolates were highly similar to each other and only one isolates showed 5% difference with other isolates. Therefore, these results show that some farmed trout in Iran are affected by Yersinia ruckeri isolates of high homology. Also, such results could be useful for protection aims such as immunization.