The effect of Fe oxide nano particles to prevention Fe chlorosis ofrootstock (citrus aurantium) in vitro condition

Iron deficiencies mostly appears as chlorosis in young leaves as one of the most important nutritional disorders is citrus trees and severely affect yield and quality of their crop. Suitable selection of iron composition is one of important solution ways for prevent of this nutritional question. For this purpose experiment to investigate the effect of iron composition type including of FeEDTA, FeEDDHA and two type of iron oxide nanoparticles uncoated and coated with polyethylene glycol for prevention iron chlorosis in sour orange (Citrus aurantium) rootstock on Murashige and Skoog (MS) medium without iron in completely randomized design with five replicates was followed. The sour orange seeds were cultivated on MS medium with different iron composition .At the end of experimental period (2 months) vegetative characteristics (root, shoot and seedling length, number and leave area), seedling, root and shoot dry and fresh weights, the amount of chlorophyll a, b and total, Leaf iron concentration and chlorosis percentage, were measured. The results indicated that MS culture medium with uncoated nanoparticles of iron oxide had highest significant effect at