Effects of plant growth regulators and culture media on shoot proliferation and reduction of vitrification of Prunus mahaleb L. via in vitro culture

Effects of different concentrations of plant growth regulators and culture media on shoot proliferation and vitrification of Prunus mahaleb L. via in vitro culture was studied using single node explants in twoseparate experiments. Shoots from single node explants were grown on 26 treatments (first experiment) of MS medium supplemented with different concentrations of cytokinins (BAP, TDZ, KIN, 2iP, and Zeatin) in combination with NAA and on 16 treatments (second experiment) of MS, 1/2 MS, WPM and DKW media supplemented with different combinations of CaCl2 and NH4NO3 and cytokinins of BAP, Zeatin, and PG. Experiments were based on completely randomized design with five replicates and each replication included 5 explants. Means comparison after 2 subcultures in the first experiment showed that the highest shoot length (3.14 cm) were obtained on 0.1 mg l-1 NAA in cytokinin -free medium, the highest number of shoot (14.67 shoot numbers per explants) on 0.2 mg l-1 Zeatin plus 0.1 mg l-1 NAA, and the lowest vitrification on 0.1 mg l-1 KIN, 0.05 and 0.1 mg l-1 Zeatin plus 0.1 mg l-1 NAA, respectively. In the second experiment, the highest shoot length (2.88 cm) were observed on DKW medium with 0.2 mg l-1 Zeatin plus 40 mg l-1 PG and the highest number of shoot (19 shoot number per explants) on MS medium with 880 mg l-1 CaCl2 and 825 mg l-1 NH4NO3. The present research by accessing to the highest proliferation (19 shoot numbers per explants) and decreasing vitrification of Prunus mahaleb L. provided a possibility of doing other research projects for protocol commercialization.