Effect of nano chitosan contain Artemisia sieberi and Satureja hortensis essences isolated on Aspergillus species from fish meal factories producing of mazandaran province

Fish meal includes valuable feeding materials along with high contents of digestible vitamins, minerals and proteins. Contamination with mycotoxins producing fungi reduce crops yields and cause irreparable damage to public health. In present study, the effect of Artemisia and Satureja nano essences on aspergillus species isolated from fish meal factories were evaluated by micro dilution in Mazandaran province. Chitosan is a non-toxic, natural, biodegradable material which is derived from chitin. Chitosan has a strong antimicrobial property against a broad spectrum of micro organisms, including fungi, a Gram-positive and Gram-negative bacteries. This study involved separation and identification of aspergillus spices in 89 samples of fish meal from 14 factories in Mazandaran province. After cultured in Sabouraud’s Dextrose Agar medium and incubation, each colony was thoroughly investigated and observed under microscope; moreover, the present aspergillusis were purified. Furthermore, the highest numbers were related to aspergillus fumigatus and aspergillus nidulance, respectively. Subsequently, to carry out the MIC, MFC test, the RPMI1640 2% culture medium was used in the micro plate The MIC test results reveal that Satureja nano essence has a better effect compared to Artemesia nano essences; in addition, the MFC test results reveal that Artemesia nano essence is more effective compared to nano essences of Satureja.