Histopathological study on sub-acute toxicity of paraquat on liver of benny fish fingerling (Barbus sharpeyi)

Agricultural pesticides and specially herbicides are one of the most important water ecosystems pollutants. Aim of this research is histopathological study of sublethal effects of Paraquat in liver of Barbus sharpeyi fingerlink. 60 fishes were divide into 3 aquarium had the following concentrations 25%, 50% and 75% of LC50 96h and one other was contained control fishes and exposed to paraquat for 96 hours. No mortalities occurred in any group during the experimental period. Then samples were fixed in 10% formalin-salin, 5μm sections were prepared and stained with haematoxylin and eosin and studied by light microscope. Histopathological lesions in exposed fishes contained nuclear conformation, pyknosis and necrosis, vacuolated cytoplasm, margination, haemorrhage in hepatic blood vessels that were sever in upper levels of paraquat. These finding showed that parraquat have toxic effects in Barbus sharpeyi and result to histopathologic lesions in liver. These observations was same with findings in other researches.