Evaluation of Antimicrobial Activity of Lactoferrin against P.Aeruginosa and E.Coli Growth

Lactoferrin(LF) is an iron-binding glycoprotein that involves a diverse range of biological activities. Lactoferrin is a major component of milk and is present in exocrine secretions such as tears, salvia, bile, and neutrophil granules. Lactoferrin has more potent antimicrobial activities against a wide range of gram negative and positive bacteria as well as antivirus activities. The purpose of this study is to evaluate the effect of this protein on P.aeruginosa growth in patients with burns that show drug resistance.
In this study, antibacterial activity of Lactoferrin has been scrutinized after isolation and purification of bovine colostrum against pseudomonas aeroginosa. Bacteria samples were isolated from scald patients (Shahid Zare Hospital); then microbial activity was confirmed with biochemical tests like oxidase, catalase and growth on TSI medium. Four concentrations 400,500,600 and 700 µg/ml of lactoferrin were assayed. Pseudomonas colonies counted and compared with negative control (without lactoferrin) as well as E.coli (DH5α) as positive control was considered.
FINDINGS: Our results showed that 400µg/ml concentration of lactoferrin has the least inhibitory effect with 35% and 29% growth inhibitory and 700µg/ml concentration of lactoferrin has the highest inhibitory effect with 86% and 66% on Pseudomonas and E.coli, respectively.
Our result showed that all of lactoferrin concentrations have inhibitory activity which in 700µg/ml has the highest inhibition against Pseudomonas aeroginosa and also E.coli.