The Protective Effect of Melatonin on Mice Fetus Testis Following lamotrigine- Induced at Pregnancy

Lamotrigine is a novel medicine that has been administrated since 1992 on few patients. There has been no comprehensive study on Organogenesis of the medicine on human. Melatonin, most important secretion of epiphysis, is an effective antioxidant and free radicals neutralizer. The present study aimed to study the removing mechanism of free radicals in testis of fetuses from mothers treated with lamotrigine using animal model as well as lamotrigine which is as an antioxidant; so, 10 mg/kg of sigma, Usa melatonin administered as peritoneal injection. At the end of pregnancy, the lengths of newborns were measured using 0.01 accuracy caliper. Their weight was measured using 0.01 gram accuracy digital weigh (Model Wtb). Then, histological studies were conducted on the newborns followed by studying under light microscope (Nikon). Data were explained as mean± SEM. data analysis was conducted using ANOVA method followed by Tukey tests in order to compare differenced among groups. According to testis histology of lamotrigine-affected group, spermatogonia destruction, spermatogonia cell's arrangement defragmenting, large vacuoles aggregation in seminiferous tubules epithelium were observed. Seminiferous tubules compression and interstitial space decrease were observed in testis of lamotrigine and melatonin compound group. Seminiferous tubules epithelial Geometric form and its arrangement were seen in a suitable condition. Morphometric and histomorphometric results of testis tissue suggested that there was a significant difference between lamotrigine group and melatonin-treated control-lamotrigine group... .