Bioremediation of n-Hexadecane Contaminated Soils Using Pseudomonas aeruginosa Bacteria Isolated from Coastal Areas

Abstract:
Background and
Purpose
Soil pollution to hydrocarbons in oil field zones in southern Iran and areas close to oil and gas transportation pipe lines is a major environmental problem. The current study aimed at determining the efficiency of bioremediation of n-Hexadecane contaminated soil as a model contaminant.
Materials And Methods
Aged hydrocarbon contaminated soils were used as the origin of bacteria isolation. Isolation of n-Hexadecane degrading bacteria was carried out through enrichment in mineral salt medium and incubation in 31 oC in shaker incubator at 150 rpm by repetitive cultures during 6 consecutive refreshing. Finally, two pure strains were isolated and identified by PCR. Bioremediation of initial n-Hexadecane concentrations of 500, 1000 and 5000 mg/kg was investigated in eight weeks.
Results
Two Pseudomonas aeruginosa strains capable of n-Hexadecane biodegradation were isolated. The removal efficiencies at the end of day 56 for n-Hexadecane initial concentrations of 500, 1000 and 5000 mg/kg were 93.6%, 87.5%, and 69.62%, respectively.
Conclusion
Isolated Pseudomonas aeruginosa bacteria demonstrated high n-Hexadecane biodegradation potential in soil and also biosurfactant production, therefore, they could be used for bioremediation of hydrocarbon contaminated soils.
Language:
Persian
Published:
Journal of Mazandaran University of Medical Sciences, Volume:26 Issue: 140, 2016
Pages:
127 to 136
https://magiran.com/p1580313  
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