Experimental study of the effect of metformin on corneal burn ulcer healing in rabbit

This study was conducted to examine the effect of metformin on corneal burn wound healing in rabbit. In this study, 20 adult healthy male New Zealand rabbits were used with an average weight of 2.5-3 kg. After general anesthesia, corneal ulcer in the left eye of the all animals was induced. After returning from anesthesia, the rabbits were randomly divided into 4 groups of 5 numbers. Then the first group was gavaged with 50 mg/kg of metformin, the second group with 100 mg/kg and the third group with 200 mg/kg while the fourth group was gavaged with water for 21 days. In the day 21 after making a corneal ulcer, tissue samples were taken and evaluated histopathologically. According to the pathological results, the vascularization, inflammation and edema of matrix of cornea significantly diminished while the epithelium of cornea increased in metformin-treated rabbits compared to control group (P