EFFECT OF ORAL ADMINISTRATION OF SALVIA RHYTIDEA EXTRACT ON THE CELLULAR NUMBERS IN CEREBELLUM FOLLOWING EXPERIMENTAL ISCHEMIA-REPERFUSION IN RAT

The salvia is one of the membranes of the Laminaceae family that its pharmacologic effects are associated to Phenolics. According to its antioxidative effect, it can remove the free O2 radicals which produced during ischemia reperfusion. In this study in order to investigate the effect of Salvia rhytidea extract on changes of cellular numbers in different layers of cerebellar cortex following ischemia and blood reperfusion in left common carotid and left vertebral arteries, 35 adult male rats were randomly divided into 7 groups of 5 rats:Group 1 (control-): Sampling whitout ischemia. Group 2 (control ): Cerebellar ischemia induced by left common carotid and left vertebral arteries occlusion with administration of normal saline. Group 3(sham): Manipulation of the left common carotid and the left vertebral arteries whitout ischemia with normal saline administration. Treatment groups (4 , 5 , 6 and 7):Cerebellar ischemia induced by the left common carotid and left vertebral arteries occlusion. Group 4 received (3.2 mg/kg) aqueous and alcoholic Salvia extract 2 hours after ischemia. Group 5 received 50 mg/kg silymarin drug, 2 hours after ischemia. Group 6 received 3.2 mg/kg aqueous and alcoholic Salvia extract 72, 48, 24 and 0 h before ischemia. Group 7 received silymarin drug (50 mg/kg), 72, 48, 24 and 0 h before ischemia. 24 h following reperfusion, the rats were euthanized, and after blood collection, samples of the cerebellum were obtained. By using routine histological technique, the sections were stained by H&E. The measurement of cell count in cerebellar cortex were accomplished.
The neuronal cell counting of cerebellum revealed that neuronal cell numbers in granular layer significantly decreased in ischemic group without treatment and in groups that received salvia extract and silymarin 2 h after ischemia compared to the control (P˂0.05).While there was no significant decrease in the number of cells of this layer in the groups that received salvia extract and silymarin before ischemia. But the cell number of molecular and purkinje layers in above groups no significant difference observed compared to the control group (P˃0.05).
Finally, according to this study, following cerebellar ischemia, the number of neuronal cells in granular layer of cerebellar cortex decreased. But pretreatment with Salvia rhytidea extract and silymarin did not change this factor. But administration of salvia extract and silymarin after ischemia, does not affect improving the number of neuronal cells in granular layer. So pretreatment with Salvia rhytidea extract silimar to silymarin drug improves the cerebellar injuries following ischemia.