The effect of exogenous quercetin and ascorbate on oat seedlings grown under different salinity levels

Environmental tensions cause increasing in active and Reactive Oxygen Species (ROS) in plants. Antioxidants materials such as querceitin and ascorbate have suitable chemical structure for free radicals deletion and increase plants resistant to tension. Oat (Avena sativa L.) have several medicinal and nutritive characteristics that can planted in saline soils by improving plant protective system against oxidative tension. The present study investigated the effect of exogenous quercetin and ascorbate separately and together on germination and antioxidant enzymes activity of Avena seedlings herb in mild and severs salinity tensions. Oat grains (promising cultivar) based on completely randomized design were treated to ascorbate in 0, 300 and 500 micro-mol, ascorbate 2 Mg -mol and NaCl in amounts of zero, 250 and 450 Mg-mol concentrations in Petri dish envirnoment. Germination percentage and antioxidant enzymes activity was evaluated in seedling. Adding quercetin and ascorbate increased germination percentage in comparison with salt in 450 Mg-mol. Adding quercitin and ascorbate the environment includes saline especially in 450 Mg-mol concentration increased peroxidase activity and decreased catalos enzyme activity. But had not significant changes in ascorbate peroxidase enzymes activity. Application of quercetin and ascorbate alone and together stimulate seed germination in high amount of salt (450 Mg-mol) that probably this reaction was due to increasing of peroxidase enzyme activity and deletion all kinds of active oxygen in Avena seedling.