Study of genes encoding resistance to cadmium and nickel in Pseudomonas aeruginosa strains isolated from wastewater Tehran

Aim and For a long time the interactions of microorganisms has been the subject of studies, and the achievements has been exploited for different usages such as: control or removal of heavy metals from wastewaters. To serve such importance the measurement of resistance of various bacteries to different heavy metals is essential.
Thus this study is oriented to assess the effects of heavy metals such as cadmium and nickel on Pseudomonas aeruginosa (extracted from wastewaters of Tehran) and exposure of encoding and resistant genes to the subject heavy metals which are similar to czr, is studied.
Material and In this study 240 specimens were collected from sewers and wastewaters of which only 10% Pseudomonas aeruginosa were extracted and purified, and thereafter the resistance of this bacterium against different densities of salt of cadmium and nickel through MIC measurement were measured and studied. Nevertheless PCR was used for the detection of encoding resistant to Cadmium (another heavy metal) gene.
The studies revealed that Pseudomonas aeruginosa Bacteria (extracted from wastewaters of Tehran) was totally immune to 1 µM of Nickel, but it was resistant to Cadmium in a range from 9.6µM to 79µM. also the existence of resistant gene to Cadmium (czr) in this Bacteria was proved via PCR.
The results showed that subject Bacteria was resistant to Cadmium, but the threshold of its resistant is dependent on the amount of heavy metal in its environment.