Effect of thyme plants on Leishmania amastigotes in invitro: compared with Amphotericin B

Leishmaniasis consisted of a set of parasitic disease with a spectrum of clinical symptoms appear, including coetaneous Leishmaniasis, mucosal and visceral all symptoms. Recently, there has been considerable progress in the use of herbal medicines for the treatment of Leishmaniasis. The purpose of this study was to evaluate the effect of thyme essential oils of four plant species compared with Amphotericin B on Leishmania Amastigotes in invitro.

Leishmania (Leishmania major) strains were cultured with medium RPMI- 1640 containing 10% fetal calf serum (FCS) and antibiotics and transported at a temperature of 25 ± 2 ° C. The stationary phase of growth were studied in different concentrations of essential oils in comparison with the control drug, Amphotericin B on Leishmania Amastigotes by using colorimetric meth. The absorbance was measured (Optical density) by means of (ELISA reader) and was calculated as the IC 50. The mean absorbance of the groups studied showed no significant difference (P

The results showed that optical absorption and IC 50 species of thyme essential oils affect drug compared with controls.

Our results showed that the herb thyme is effective for the treatment of coetaneous Leishmaniasis. According to these results suggest that the efficacy was evaluated alone or in combination against human coetaneous Leishmaniasis as a randomized clinical trial.