Tracing the Enterocin Encoding Structural Genes in Enterococcus Isolated From Kurdish Traditional Cheese

Enterocins are those bacteriocins that produce by Enterococcus strains. Enterocins have a large variety and any strain of Enterococcus, according to their genetic content and growth environment can produce type or types of Enterocin. In the present study 15 bacterial strains (Enterococcus genus) chose that had isolatated of Kurdish traditional cheese bye cultured-based and molecular methods. Genomic DNA isolation VI kit that was specific for Gram-positive bacteria with hard well, was used to DNA extraction of isolates. Polymerase chain reaction was performed, in order to tracing the Enterocin encoding structural genes. The results showed that the Enterocin gene A was 10 of 15 isolates and had the highest frequency and after the highest frequency was related to Enterocin gene P with 9 cases and B with 8 cases, respectively. The majority of investigated Enterococcus isolates in this study had Enterocin producing genes. Enterococcus faecalis NRIC0112 and Enterococcus faecalis SK13 had 5 different genes, Enterocin A, Enterocin B, Enterocin P, Enterocin 31 and Enterocin X. 1071, L50, AS48 and KS Enterocin genes was not found in any of the isolates. However, the use of these isolates in industrial and dairy products demands more researches.