In vitro Shoot Regeneration of Medicinal Plant Anthemis hyalina DC

Anthemis hyalina DC. is one of the most important herbal medicine plants and is used for anti-allergy and curing skin rashes or acne. To date there is no report for in vitro culture of this species and the present study was performed for the first time with the aim of obtaining the optimized treatment for shoot regeneration. In this research the effect of different plant growth regulators NAA (0, 0.1, 0.5, 1.5 mg L-1) and BA (0, 1, 3 mg L-1) on callus induction and shoot regeneration in leaf and immature embryo explants was investigated. This experiment has been carried out as factorial in a completely randomized design with four replications. The results showed the significant effects of all main factors and interactions on measured traits in 0.01 probability level. The highest frequency of shoot regeneration for leaf explant was observed on the medium containing 1 mg L-1 BA. In embryo explant the best treatment for shoot regeneration was 3 mg L-1 BA.