Evaluation of Genetic diversity Rose (Rosa hybrid) flower genotypes using CDDP & RAPD markers

Identification of genetic diversity and classification of genetic resources (germplasm) are of important and essential activities in breeding and management of plant genetic resources. In order to study the genetic diversity of rose, twenty genotypes were analyzed using six Conserved DNA-Derived Polymorphism (CDDP) and 20 RAPD markers. Matrix genetic distance ranged from 0.04 to 0.77 in CDDP and from 0.05 to 0.79 in RAPD marker analysis. The level of polymorphism generated by CDDP markers (100%) was similar to RAPD marker. Cluster analysis for CDDP and RAPD markers revealed that genotypes were grouped in three clusters. The current study showed CDDP marker very well differentiated rose genotypes from each other. This is the first report of using targeted DNA region molecular marker (CDDP) for genetic diversity analysis in rose in comparison with RAPD markers. We introduce CDDP as a new molecular markers system for evaluating Rose germplasm.